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University of Concepcion

Biospectroscopy & Chemometrics Laboratory, Biotechnology Center, Chile

Our research is based on Analytical Chemistry applications in areas such as vegetal biotechnology, renewable resources and pharmaceuticals analysis. We develop analytical strategies based on vibrational spectroscopy and other molecular spectroscopies, as well as modern microscopy techniques and hyperspectral imaging, supported with multivariate analysis (Chemometrics).

Our Team

Dr. Rosario Castillo

Experienced Researcher

Dr. Mario Sanhueza

Experienced Researcher

Macarena Rojas

Early Stage Researcher

Cristian Fuentes

Early Stage Researcher

Martín Bravo

Early Stage Researcher


  • Angelo Beratto-Ramos, Cristian Agurto-Muñoz, Juan Pablo Vargas-Montalba, Rosario del P. Castillo*. Fourier-transform infrared imaging and multivariate analysis for direct identification of principal polysaccharides in brown seaweeds. Carbohydrate Polymers, 2020, 115561,
  • Sanhueza, MI, Meléndrez, MF, von Plessing, C, Neira José, Y. Freer J, Castillo R del P*. Raman microimaging as an analytical technique for simultaneous quantification and localization of active principles in pharmaceutical solid dosage forms. J Raman Spectrosc. 2020; 51: 649– 659.
  • Ángela Álvarez, Jorge Yáñez, Yamil Neira, Rosario Castillo-Felices, Patricio Hinrichsen. Simple distinction of grapevine (Vitis vinifera L.) genotypes by direct ATR-FTIR. Food Chemistry, 2020: 328, 127164. FI:6.306.
  • Toro, C*.; Torres, S.; Parra, V.; Fuentes, R.; Castillo, R.; Díaz, W.; Reyes, G.; Balladares, E.; Parra, R. On the Detection of Spectral Emissions of Iron Oxides in Combustion Experiments of Pyrite Concentrates. Sensors 202020, 1284.
  • César Pino-Torres, Santiago Maspoch, Rosario Castillo-Felices, Mónica Pérez-Rivera, Mario Aranda-Bustos, Carlos Peña-Farfal*. Evaluation of NIR and Raman spectroscopies for the quality analytical control of a solid pharmaceutical formulation with three active ingredients. Microchemical Journal, 2020, 154,104576.
  • Felipe Aravena-Sanhueza, Mónica Pérez-Rivera, Rosario Castillo-Felices, Rodolfo Mundaca-Uribe, Mario Aranda Bustos and Carlos Peña Farfal. Determination of antioxidant capacity (ORAC) of Greigia sphacelata and correlation with voltammetric methods. 2020. Journal of the Chilean Chemical Society 65(3):4925-4928.
  • Analytical strategies based on near infrared spectroscopy and multivariate calibration for rapid quantification of florfenicol at low-concentrations in medicated-feed pellets. Camila Y. Bastidas, Carlos von Plessing, José Troncoso, Mario I. Sanhueza, José Y. Neira, Rosario del P. Castillo*. Microchemical Journal,2019, 147: 1068-1074.
  • Juan A. Araya*, Renato L. Carneiro, Juanita Freer, José Y. Neira, Rosario del P. Castillo. Fourier transform infrared imaging and quantitative analysis of pre-treated wood fibers: A comparison between partial least squares and multivariate curve resolution with alternating least squares methods in a case study. Chemometrics and Intelligent Laboratory Systems. 2019, 195: 103890,
  • Determination of copper-based mineral species by laser induced breakdown spectroscopy and chemometric methods† Jonnathan Alvarez,Marizu Velasquez, Ashwin Kumar Myakalwar, Claudio Sandoval, Rodrigo Fuentes, Rosario Castillo, Daniel Sbarbaro and Jorge Yañez*. Journal of Analytical Atomic Spectrometry, 2019. DOI: 10.1039/c9ja00271e.
  • Valentina Manzo, Jairón Goya-Pacheco, Daniel Arismendi, Mercedes Becerra-Herrera, Alver Castillo-Aguirre, Rosario Castillo-Felices, Milton Rosero-Moreano, Eduardo Carasek, Pablo Richter*. Cork sheet as a sorptive phase to extract hormones from water by rotating-disk sorptive extraction (RDSE). Analytica Chimica Acta. 2019, 1087: 1-10.
  • Mendez, Paola, Christian Nuñez, Jaime  Cabrera-Pardo , Cristian Paz, Juan Manuel Barraza, Rosario Castillo and Carlos Peña-Farfal*. Adsorption ability of activated carbon obtained from sub-bituminous coal (lebu, chile) to capture trimethylamine. J. Chil. Chem. Soc. 2019, vol.64, n.3, pp.4582-4585. Issn 0717-9707.  Http://
  • Multivariate calibration for the improvement of the quantification of cadmium in the presence of potassium as interferent by total reflection X‐ray fluorescence. Medina González, G*, Castillo, R d P,  Neira, JY.  X‐Ray Spectrometry. 2019; 48: 700– 707.


  • Evaluation of the performance of confocal laser-scanning fluorescence microscopy (CLSFM) enhanced by advanced chemometric methods for direct solid analysis of pharmaceutical forms and samples with high topochemistry functionality dependence. Fondecyt Regular 1191281, ANID, Chile (2019-2022). Responsible Researcher: Rosario del P. Castillo.